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Tumor Profiling

Learning the molecular pixels in a tumor

Cellular heterogeneity and perturbation of spatial relationships have been implicated in various physiological and pathological conditions. Cancer migration and metastasis are often led by a subset of aggressive cells, which emerge elusively, with genotypic and phenotypic plasticity in their repertoire. Yet, there is still a knowledge gap in the spatial determinants due to the challenge of integrating single-cell, multi-omic and spatial analysis on the same sample.


To capitalize clinically on new insights gleaned from preliminary single-cell + spatial biology studies, there is an urgency for technologies that democratize and disseminate these methods within the cancer research community. In this project, our focus is on creating multiomic spatial profiling workflows for tumor tissue sections using our open-space MFP technology, using its capability of simultaneous deposition (tag) and lysis (bag) of cells in tissues.

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Current Projects
Tag & Bag

Tag & Bag aims to implement spatial barcoding approaches in concert with lysis using microfluidic delivery of oligo barcoding tags. By leveraging improved mass transfer and reaction rates conferred by HFCs and user-defined microscale localization, our aim is to selectively tag ROIs on tissue sections and performing multiplexed assays on unprocessed tissues.

Cell 2 Spec

Cell 2 Spec aims to implement selective local lysis (SpatiaLyse) on live cells and tissue sections to enable interfacing with mass spectrometry (MS). Using viscous shear, and detergent solubilization coupled with controlled enzymatic lysis, we aim to perform MS-sample prep spatially using the MFP.

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Our Workflows
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Related Publications

Space in cancer biology: its role and implications

Trends in Cancer

Mapping spatial genetic landscapes in tissue sections through microscale integration of sampling methodology into genomic workflows


A compact and versatile microfluidic probe for local processing of tissue sections and biological specimens

Review of Scientific Instruments

Quantification of tumor heterogeneity: from data acquisition to metric generation

Trends in Biotechnology

Spatially resolved genetic analysis of tissue sections enabled by microscale flow confinement retrieval and isotachophoretic purification

Angewandte Chemie International Edition

Micro-immunohistochemistry using a microfluidic probe

Lab on a Chip

Spatial protein heterogeneity analysis in frozen tissues to evaluate tumor heterogeneity


Tissue lithography: microscale dewaxing to enable retrospective studies on formalin-fixed paraffin-embedded (FFPE) tissue sections


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